Дополнительная информация
1. Финансовая поддержка исследований (2015–2020 год):
РНФ №18–15-00106 (рук. Ю. А. Негуляев)
РНФ №19–75-00046 (рук. В. И. Чубинский-Надеждин)
РНФ №17–74-10123 (рук. В. И. Чубинский-Надеждин)
РФФИ №19–015-00211а (рук. А. В. Сударикова)
РФФИ №18–04-00231а (рук. С. Б. Семенова)
РФФИ №16–04-00467а (рук. Ю. А. Негуляев)
РФФИ №15–34-20464 мол_а_вед (рук. В. И. Чубинский-Надеждин)
РФФИ-15–04-00938а (рук. С.Б. Семенова)
РФФИ №15–04-002950а (рук. Е. А. Морачевская)
РФФИ №15–04-00905а (рук. В. И. Чубинский-Надеждин)
РНФ 14–50-00068 “Реализация комплексных научных программ организаций” (участие, 2014–2018)
Стипендии Президента РФ для аспирантов и молодых ученых (2013–2015, 2015–2017) Гранты Санкт-Петербурга в сфере научной и научно-технической деятельности
Программа “Молекулярная и Клеточная Биология” (участие, рук. акад. Н. Н. Никольский)
2. Подготовка научных кадров:
Негуляев Ю.А., Морачевская Е.А. – члены Диссертационного совета Д 002.230.01 при Институте цитологии РАН.
Негуляев Ю. А. – Лекции для студентов Петербургского политехнического университета Петра Великого с 01.09.1998 по 30.06.2002 в качестве доцента, а с 01.09.2003 по настоящее время в качестве профессора. В разное время читал курсы «Современные методы биофизики» и «Структура клеточных компартментов». В настоящее время «Ионные механизмы клеточной сигнализации» для студентов кафедры медицинской физики.
3. Научное сотрудничество:
Medical University of South Carolina (Чарльстон, Южная Каролина, США).
Институт Биоорганической химии им. М. М. Шемякина и Ю. А. Овчинникова РАН (Москва).
Medical College of Wisconsin (Милуоки, Висконсин, США).
University of Texas Health Science Center, Houston, Texas, USA.
4. Наши коллеги – бывшие сотрудники и ученики:
Васильева И. О., Городская Больница 31, Лаборатория Молекулярной Генетики, СПб.
Шуйский Л. С., лаб. Ионных Каналов Клеточных Мембран, ИНЦ РАН
Томилин В. Н., University of Texas Health Science Center, Houston, Texas, USA.
Илатовская Д. В., Medical University of South Carolina, USA.
Шумилина Е. В., University of Tubingen, Germany
Старущенко А. В., Medical College of Wisconsin, USA
Максимов А. В., Scripps Research Institute, California, USA.
5. Избранные публикации (до 2015 г) – Фундамент наших исследований и текущих проектов :
Ilatovskaya DV, Palygin O, Chubinskiy-Nadezhdin V, Negulyaev YA, Ma R, Birnbaumer L, Staruschenko A. Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli. Kidney International, 2014, 86(3):506–514.
Chubinskiy-Nadezhdin VI, Negulyaev YA, Morachevskaya EA. Functional coupling of ion channels in cellular mechanotransduction. Biochemical and Biophysical Research Communications, 2014. 451(3):421–424.
Ilatovskaya DV, Chubinskiy-Nadezhdin V, Shuyskiy LS, Tomilin V, Pavlov TS, Palygin O, Staruschenko A and Negulyaev YA. Arp2/3 complex inhibitors adversely affect actin cytoskeleton remodeling in the cultured murine kidney collecting duct M‑1 cells. Cell and Tissue Research, 2013, Sep 15, 354(3): 783–792.
Chubinskiy-Nadezhdin VI, Efremova TN, Khaitlina SY and Morachevskaya EA. Functional impact of cholesterol sequestration on actin cytoskeleton in normal and transformed fibroblasts. Cell Biol. Int. 2013; 37: 617–623.
Ilatovskaya DV, Staruschenko A. Single channel analysis of TRPC channels in the podocytes of the freshly isolated glomeruli. From: Methods in Molecular Biology. Ion Channels: Methods and Protocols. 2013, Humana Press Inc. 998:355–69, PMID: 23529444.
Vassilieva IO, Tomilin VN, Marakhova II, Shatrova AN, Negulyaev YA, Semenova SB. Expression of Transient Receptor Potential Vanilloid Channels TRPV5 and TRPV6 in Human Blood Lymphocytes and Jurkat Leukemia T Cells. The Journal of Membrane Biology. 2013, 246 (2): 131–40.
Ilatovskaya DV, Pavlov TS, Levchenko V, Negulyaev YA, Staruschenko AV. Cortical actin binding protein cortactin mediates ENaC activity via Arp2/3 complex. FASEB J. 2011, 25(8), 2688–99.
Chubinskiy-Nadezhdin VI, Negulyaev YA, Morachevskaya EA. Cholesterol depletion-induced inhibition of stretch-activated channels is mediated via actin rearrangement. Biochemical and Biophysical Research Communications 2011, V.412(1), 80–5.
Karpushev AV, Ilatovskaya DV, Pavlov TS, Negulyaev YA, Staruschenko AV. Intact cytoskeleton is required for small G protein dependent activation of the epithelial Na+ channel. PLoS One. 2010, V.5(1), e8827.
Semenova SB, Vassilieva IO, Fomina AF, Runov AL, Negulyaev YA. Endogenous expression of TRPV5 and TRPV6 calcium channels in human leukemia K562 cells. Am J Physiol Cell Physiol. 2009, 296 (5), 1098–1104.
Sviderskaya EV, Easty DJ, Lawrence MA, Sanchez DP, Negulyaev YA, Patel RH, Anand P, Korchev YE, Bennett DC. Functional neurons and melanocytes induced from immortal lines of postnatal neural crest-like stem cells. FASEB J. 2009, 23 (9), 3179–3192.
Gorelik J, Ali N. A., Siti H. Sheikh Abdul Kadir, Max Lab, Stojkovic P., Armstrong L, Sviderskaya E. V., Yuri A. Negulyaev, Klenerman D, Bennett D. C., Lako M., Harding S. E., Stojkovic M. and Yuri E. Korchev. Non-invasive Imaging of Stem Cells by Scanning Ion Conductance Microscopy: Future Perspective. TISSUE ENGINEERING: Part C. 2008, 14 (4), 311–318.
Morachevskaya EA, Sudarikova AV, Negulyaev YA. Mechanosensitive channel activity and F‑actin organization in cholesterol-depleted human leukaemia cells. Cell Biology International. 2007, 31, 374–381.
Staruschenko AV, Sudarikova AV, Negulyaev YA, Morachevskaya EA. Magnesium permeation through mechanosensitive channels: single-current measurements. Cell Research. 2006, 16 (8), 723–730.
Semenova SB, Fomina AF, Vassilieva IO, Negulyaev YA. Properties of Mg2+-dependent cation channels in human leukemia K562 cells. Journal of Cellular Physiology. 2005, 205 (3), 372–378.
Staruschenko AV, Negulyaev YA, Morachevskaya EA. Actin cytoskeleton disassembly affects conductive properties of stretch-activated cation channels in leukaemia cells. Biochimica et biophysica acta. 2005, 1669 (1), 53–60.
Shumilina EV, Khaitlina SY, Morachevskaya EA, Negulyaev YA. Non-hydrolyzable analog of GTP induces activity of Na+ channels via disassembly of cortical actin cytoskeleton. FEBS Letters. 2003 July 17, 547(1–3), 27–31.
Shumilina EV, Negulyaev YA, Morachevskaya EA, Hinssen H, Khaitlina SY. Regulation of sodium channel activity by capping of actin filaments. Molecular Biology of the Cell. 2003, 14 (4), 1709–1716.
Staruschenko AV, Vedernikova EA. (Morachevskaya EA). Mechanosensitive cation channels in human leukaemia cells: calcium permeation and blocking effect. Journal of Physiology. 2002, 541, 81–90.
Negulyaev YA, Khaitlina SY, Hinssen H, Shumilina EV, Vedernikova EA. (Morachevskaya EA.) Sodium channel activity in leukemia cells is directly controlled by actin polymerization. The Journal of Biological Chemistry. 2000, 275 (52), 40933–7.
Korchev YE, Negulyaev YA, Edwards CR, Vodyanoy I, Lab MJ. Functional localization of single active ion channels on the surface of a living cell. Nature Cell Biology. 2000, 2 (9), 616–619.
Negulyaev YA, Markwardt F. Block by extracellular Mg2+ of single human purinergic P2X4 receptor channels expressed in human embryonic kidney cells. Neuroscience Letters. 2000, 279 (3), 165–168.
Ведерникова Е. А. (Морачевская Е.А), Максимов А. В., Негуляев Ю.А. Функциональная характеристика и молекулярная топология потенциал-независимых натриевых каналов. Цитология. 1999, 41 (8), 658–666.
Maximov AV, Vedernikova EA (Morachevskaya EA), Hinssen H, Khaitlina SY, Negulyaev YA. Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS Lett. 1997, Jul 21, 412 (1), 94–96.
Negulyaev, Y.A., Maximov A.V., Vedernikova E.A. (Morachevskaya EA), and Katina I.E.. Voltage-insensitive Na channels of different selectivity in human leukemic cells. General Physiology and Biophysics. 1997, 16, 163–173.
Negulyaev YA, Vedernikova EA (Morachevskaya EA), Maximov AV. Disruption of actin filaments increases the activity of sodium-conducting channels in human myeloid leukemia cells. Molecular Biology of the Cell. 1996, 7 (12), 1857–64.
Negulyaev, Y.A., Vedernikova E.A. (Morachevskaya EA), Kinev A.V., and Voronin A.P. Exogenous heat shock protein hsp70 activates potassium channels in U937 cells. Biochimica et Biophysica Acta. 1996, 1282, 156–162.
Negulyaev YuA, Vedernikova EA (Morachevskaya EA), Mozhayeva GN. Several types of sodium-conducting channel in human carcinoma A‑431 cells. Biochimica et Biophysica Acta. 1994, 1194 (1), 171–175.
Negulyaev YA, Vedernikova EA. (Morachevskaya EA). Sodium-selective channels in membranes of rat macrophages. The Journal of Membrane Biology. 1994, 138 (1), 37–45.